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This project aimed to identify the causative agent of Spotty Liver Disease (SLD) by both molecular and in vitro techniques, and to assess treatment and control options in the field. The study involved a literature review, and the investigation of field cases and sampling from both affected birds and those in another shed on the same farm, which were not affected. These samples were used to examine the histopathology of affected birds, to undertake genetic analysis of the gut flora of affected and control birds, to examine possible cell-toxicity of sera from affected birds, and to undertake challenge studies to attempt to reproduce the disease.
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