Showing 3 of 3 results for "PCR"
This study was conducted to develop and evaluate the use of reverse transcriptase real time polymerase chain reaction (RT-PCR) to detect and quantify the viral copy number of infectious bronchitis virus (IBV) from infected tissues of vaccinated and unvaccinated laying hens. The real-time PCR test designed during this project is highly sensitive and can detect as low as 10 viral copy numbers. Along with virulent ‘T’ and vaccine strains A3 and Vic S, the test will also be able to detect at least seven other Australian IBV strains.
This project conducted laboratory and in-field experiments to fully test and validate the molecular diagnostic tests used to differentiate between wild type and Rispens CVI988 serotype 1 Marek’s disease virus (MDV). It also investigated the spread of Rispens between chickens and the effect of wild-type MDV challenge at different times post vaccination on the level of protection provided by Rispens vaccination. Results of this study expands understanding of the interaction between pathogenic and vaccinal viruses following vaccination with imperfect vaccines and provides advice for selection of appropriate samples to test for vaccination success.
Development of Molecular Tests for Serovar-Specific Identification and Typing of Haemophilus paragallinarum
Haemophilus paragallinarum is the causative agent of infectious coryza, an upper respiratory tract disease of chickens characterised by relatively mild clinical signs. As there have been few studies on methods to type isolates of H. Paragallinarum, this project set to evaluate two different types of rapid, DNA based typing methods.
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